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Further large-scale RCTs are required to confirm GSHs efficacy, determine optimal dosing, and assess long-term effects
Subsequently, the cells were fixed with 4% paraformaldehyde at 16 C for 15 min, permeabilized in phosphate buffered saline (PBS) with 0.1% Triton X-100 for 5 min at 16 C, and incubated with first antibodies at 4 C overnight, followed by incubation with FITC-conjugated secondary antibodies at 16 C for 1 h
However, individual responses may vary
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